Test Methods

The availability of standard, validated and easy-to-run methods for resistance detection in the world’s major insect pests is crucial for successful monitoring of resistance problems. Shown below are the IRAC Approved Test Methods which have been validated by IRAC member companies and collaborators to ensure that they provide reliable and reproducible data.

Most tests are specific to particular life-history stages and can only be used with confidence for toxicants which have been validated in the development of the methodology.  For more information please visit the IRAC Methods Team page.

NumberVideoSpeciesStageMethodologyModes of Action
001 Has video
  • Green peach aphid Myzus persicae
  • All Stages of Development
  • Dip
View Test Method 001
002
  • Psyllids Psylla spp.
  • All Stages of Development
  • Dip
View Test Method 002
003
  • European Red Mite Panonychus ulmi
  • Twospotted Spider Mite Tetranychus urticae
  • Eggs
  • Dip
View Test Method 003
004
  • Citrus red spider mite Panonychus citri
  • European Red Mite Panonychus ulmi
  • Twospotted Spider Mite Tetranychus urticae
  • Adults
  • Dip
View Test Method 004
005 Has video
  • Rice green leafhopper Nephotettix cincticeps
  • Brown planthopper Nilaparvata lugens
  • Adults
  • Dip
View Test Method 005
006
  • Red flour beetle Tribolium castaneum
  • All Stages of Development
  • Filter-paper
View Test Method 006
007
  • Corn earworm Helicoverpa zea
  • Tobacco budworm Heliothis virescens
  • Pseudoplusia includens
  • Adults
  • Larvae
  • Dip
View Test Method 007
008
  • Tobacco Whitefly Bemisia tabaci
  • Adults
  • Dip
View Test Method 008
009
  • Pear Leaf Blister Moth Leucoptera scitella
  • Apple leaf miner moth Lithocolletis blancaedella
  • Larvae
  • Eggs
  • Dip
View Test Method 009
010 Has video
  • Western flower thrips Frankliniella occidentalis
  • Adults
  • Dip
View Test Method 010
011 Has video
  • Pollen Beetle Meligethes aeneus
  • Adults
  • Vial
View Test Method 011
012
  • European Red Mite Panonychus ulmi
  • Adults
  • Petri-dish
View Test Method 012
013
  • European Red Mite Panonychus ulmi
  • Adults
  • Dip
View Test Method 013
014
  • Western flower thrips Frankliniella occidentalis
  • Larvae
  • Dip
View Test Method 014
015
  • Tobacco Whitefly Bemisia tabaci
  • Glasshouse whitefly Trialeurodes vaporariorum
  • Adults
  • Dip
View Test Method 015
016
  • Tobacco Whitefly Bemisia tabaci
  • Glasshouse whitefly Trialeurodes vaporariorum
  • Nymphs
  • Eggs
  • Dip
View Test Method 016
017
  • Codling Moth Cydia pomonella
  • Larvae
  • Diet
View Test Method 017
018
  • Diamondback Moth Plutella xylostella
  • Larvae
  • Dip
View Test Method 018
019 Has video
  • Pea aphid Acyrthosiphon pisum
  • Black bean aphid Aphis fabae
  • Soybean aphid Aphis glycines
  • Melon & Cotton Aphid Aphis gossypii
  • Buckthorn Aphid Aphis nasturtii
  • Foxglove aphid Aulacorthum solani
  • Potato Aphid Macrosiphum euphorbiae
  • Rose-grain aphid Metopolophium dirhodum
  • Green peach aphid Myzus persicae
  • Currant-lettuce aphid Nasonovia ribisnigri
  • English grain aphid Sitobion avenae
  • Adults
  • Nymphs
  • Dip
View Test Method 019
020
  • Corn earworm Helicoverpa zea
  • Tobacco budworm Heliothis virescens
  • Southern armyworm Spodoptera eridania
  • Beet armyworm Spodoptera exigua
  • Fall armyworm Spodoptera frugiperda
  • Cotton leafworm Spodoptera littoralis
  • Cotton leafworm Spodoptera litura
  • Larvae
  • Diet
View Test Method 020
021 Has video
  • Pollen Beetle Meligethes aeneus
  • Adults
  • Vial
View Test Method 021
022 Has video
  • Tomato leafminer Tuta absoluta
  • Dip
View Test Method 022
023
  • Green peach aphid Myzus persicae
  • Nymphs
  • Feeding
View Test Method 023
024
  • Melon & Cotton Aphid Aphis gossypii
  • Adults
  • Nymphs
  • Feeding
View Test Method 024
025 Has video
  • Pollen Beetle Meligethes aeneus
  • Adults
  • Vial
View Test Method 025
026
  • Housefly Musca domestica
  • Adults
  • Feeding
View Test Method 026
027 Has video
  • Pollen Beetle Meligethes aeneus
  • Adults
  • Vial
View Test Method 027
028
  • Stink Bug Euschistus heros
  • Adults
  • Dip
View Test Method 028
029
  • Stink Bug Euschistus heros
  • Adults
  • Topical
View Test Method 029
030 Has video
  • Stink Bug Euschistus heros
  • Adults
  • Vial
View Test Method 030
031
  • Oilseed rape stem weevil Ceutorhynchus napi
  • Ceutorhynchus obstrictus
  • Cabbage leaf weevil Ceutorhynchus pallidactylus
  • Cabbage Flea Beetles Phyllotreta spp
  • Cabbage stem flea beetle Psylliodes chrysocephala
  • Adults
  • Vial
View Test Method 031
032
  • Asian Citrus Psyllid Diaphorina citri
  • Nymphs
  • Dip
View Test Method 032
033
  • Western Tarnished Plant Bug Lygus hesperus
  • Adults
  • Nymphs
  • Dip
View Test Method 033
101
  • Southwestern Corn Borer Diatraea grandiosella
  • Sugarcane Borer Diatraea saccharalis
  • European Corn Borer Ostrinia nubilalis
  • Pink Borer Sesamia inferens
  • Larvae
View Test Method 101